(FMDV-A Ab) Uhlobo lweNtsholongwane yeSifo sonyawo-nomlomo iKhiti yoVavanyo lwe-Antibody
[Ngasemva]
Imveliso inokumisela ngokukhawuleza imeko yesifo sonyawo kunye nomlomo wentsholongwane yohlobo lwe-A-antibodies kwizilwanyana ngokusebenzisa iimvavanyo ze-immunochromatographic kwi-serum okanye iisampulu zeplasma kunye nokufunyanwa kwesifo sonyawo kunye nomlomo wentsholongwane yesifo sohlobo lwe-A-antibodies ethile, ukuze unikeze ireferensi yeklinikhi. ukuxilongwa kwentsholongwane yesifo sonyawo kunye nomlomo uhlobo A.
[Umgaqo-siseko]
Le mveliso isebenzisa i-immunochromatography ekhawulezayo yokukhangela intsholongwane yesifo sonyawo kunye nomlomo we-A antibodies.Emva kokuba isampulu yongezwa kumngxuma wesampulu ye-adde yesampulu yekhadi lobhaqo, ukuba uhlobo lwentsholongwane yesifo sonyawo kunye nomlomo lukhona kwisampulu, i-antibody inokudibanisa ngokuthe ngqo kunye nentsholongwane ye-foot-and-mouth virus uhlobo lwe-antigen ephawulweyo. ngegolide eyicolloidal ukwenza ikhompawundi ehamba ecaleni kwenwebu yechromatographic kwaye ithinjwe yiproteni eqatywe ngaphambili kwinwebu yechromatographic ukwenza umgca wokufumanisa obomvu wewayini kwindawo t yeqokobhe leclam.Ngokungabikho kwe-FMDV yohlobo lwe-antibody kwisampuli, akukho mgca obonakalayo owenziwe kwindawo ye-T.Ukongeza, umgca we-C waphinda wayilwa kule nkqubo ukuqinisekisa ukusebenza kakuhle kovavanyo.Umgca uya kufakwa umbala nokuba uyala okanye uphozithivu, kungenjalo uya kubhengezwa njengongasebenziyo.
[Ukwakheka kwemveliso]
Uhlobo lwentsholongwane yesifo sonyawo nomlomo Ikiti yovavanyo lwe-antibody (iingxowa ezingama-50/ibhokisi)
Idropper (1 pc/bag)
I-Desiccant (1pc/bag)
Umyalelo (1 pc/ibhokisi)
[Ukusetyenziswa]
Nceda ufunde imiyalelo yokusebenza ngononophelo phambi kovavanyo, kwaye ubuyisele ikhadi lovavanyo kunye nesampulu ukuba ivavanywe kwiqondo lobushushu begumbi le-15–25℃.
1. Igazi elitsha laqokelelwa, i-serum yahlulwe ngokuma, okanye iisampuli zeplasma zafunyanwa nge-centrifugation, kwaye iisampulu zaqinisekiswa ukuba zingabi namafu okanye zigqithise.
2. Khupha iqhekeza lepokotho yekhadi lovavanyo kwaye uyikrazule, khupha ikhadi lovavanyo, lenze lilingane kwiqonga lomsebenzi.
3. Kwisampula kakuhle "S", yongeza i-2-3 yehla (malunga ne-70-100 mL) yesampuli.
4. Uqwalaselo kwimizuzu emi-5-10, alusebenzi emva kwemizuzu eli-15.
[Isiphumo isigwebo]
* Okuhle (+): Iibhanti zewayini ezibomvu zomgca wolawulo C kunye nomgca wokufumanisa u-T ubonise ukuba isampulu iqulethe isifo sonyawo kunye nomlomo sohlobo A lwe-antibody.
* Engalunganga (-): Akukho mbala uphuhliswe kwi-T-reyi yovavanyo, ebonisa ukuba isampulu yayingenayo i-anti-and-mouth disease type A antibody.
* Ayisebenzi: Akukho QC Line C okanye Whiteboard ekhoyo ebonisa inkqubo engalunganga okanye ikhadi elingasebenziyo.Nceda uphinde uvavanye.
[Ukulumkela]
1. Nceda usebenzise ikhadi lovavanyo ngaphakathi kwexesha lesiqinisekiso kwaye kwiyure enye emva kokuvula:
2. Xa uvavanya ukuphepha ukukhanya kwelanga kunye nokuvuthela kwefeni yombane;
3. Zama ukuba ungachukumisi ifilimu emhlophe embindini wekhadi lokubona;
4. I-dropper yesampuli ayinakuxutywa, ukwenzela ukuba ugweme ukungcoliswa komnqamlezo;
5. Musa ukusebenzisa isampulu yedilunti engabonelelwanga nale reagent;
6. Emva kokusetyenziswa kwekhadi lokubhaqa kufuneka lithathwe njengokulungiswa kwempahla enobungozi yemicrobial;
[Unyino lwesicelo]
Le mveliso yikiti yokuxilonga i-immunological kwaye isetyenziselwa ukubonelela ngeziphumo zovavanyo olusemgangathweni ukubhaqwa kwezonyango zezifo zezilwanyana zasekhaya.Ukuba kukho amathandabuzo malunga neziphumo zovavanyo, nceda usebenzise ezinye iindlela zokuxilonga (ezifana ne-PCR, uvavanyo lwe-pathogen isolation, njl.njl.) ukwenza uhlalutyo olongezelelweyo kunye nokuxilongwa kweesampuli ezichongiweyo.Qhagamshelana nogqirha wezilwanyana wengingqi yakho malunga nohlalutyo lwezifo.
[Ugcino kunye nokuphelelwa]
Le mveliso kufuneka igcinwe ku-2 ℃–40℃ kwindawo epholileyo, eyomileyo kude nokukhanya kwaye ingakhenkcezi;Isebenza iinyanga ezingama-24.Jonga iphakheji yangaphandle yomhla wokuphelelwa kunye nenombolo yebhetshi.
