IKhiti yoVavanyo ye-Classical Swine Fever Virus Antibody
[Ngasemva]
Ukuveliswa kuveza ikhadi lentsholongwane yehagu yehagu ekhawulezayo yokufumanisa intsholongwane yehagu, eliyindlela enqamlezileyo yovavanyo lwe-immunochromatographic yokufunyaniswa komgangatho we-classical swine fever virus antibody (CSFV Ab) kwiserum yehagu okanye iisampulu zeplasma.
Ixesha lovavanyo: 5- 10 imizuzu
Iisampulu: i-serum kunye neesampuli zeplasma.
[Umgaqo-siseko]
Le mveliso isebenzisa i-immunochromatography assay ekhawulezayo yokukhangela amajoni omzimba kwintsholongwane ye-classical swine fever.Isampulu i-adde kwisampulu eyongeza umngxuma welo khadi lobhaqo, kwaye ukuba kukho i-antibody yentsholongwane yomkhuhlane wehagu kwisampulu, i-antibody inokudibanisa ngokukodwa i-antigen ebhalwe ngegolide ye-colloidal ukwenza ikhompawundi ehamba ecaleni kwenwebu yechromatographic kwaye ibanjwe yi-antibody egqunyiweyo yentsholongwane yehagu kwi-chromatographic inwebu ukwenza umgca wobhaqo wewayini kwindawo yeqokobhe lembaza t.Isetyenziselwa ukujonga ukubaleka kovavanyo kunye nofundo lweziphumo.Ukungabikho kwe-antibody ye-virus yehagu kwisampulu, akukho mgca ubonakalayo owenziwe kwindawo ye-T.Ukongeza, umgca we-C waphinda wayilwa kule nkqubo ukuqinisekisa ukusebenza kakuhle kovavanyo.Umgca uya kufakwa umbala nokuba uyala okanye uphozithivu, kungenjalo uya kubhengezwa njengongasebenziyo.
[Ukwakheka kwemveliso]
Ikhithi yokuvavanya i-antibody fever yehagu (iingxowa ezingama-50/ ibhokisi) Idropha ( 1 pc/bag)
I-Desiccant (1 pc/bag)
Diluent (50 iibhotile/ibhokisi, 1.0mL/ ibhotile)
Umyalelo (1 pc/ibhokisi)
[Ukusetyenziswa]
Nceda ufunde imiyalelo yokusebenza ngononophelo phambi kovavanyo, kwaye ubuyisele ikhadi lovavanyo kunye nesampulu ukuba ivavanywe kwiqondo lobushushu begumbi le-15–25℃.
1. Qokelela i-serum entsha okanye iisampulu zeplasma kwaye uqinisekise ukuba iisampulu azinayo i-turbidity okanye intlenga.
2. Khupha iqhekeza lepokotho yekhadi lovavanyo kwaye uyikrazule, khupha ikhadi lovavanyo, lenze lilingane kwiqonga lomsebenzi.
3. Yongeza kancinane i-2-3 yehla (malunga ne-80- 100uL) ye-serum okanye i-plasma kwiSampuli ye-Well Well "S" usebenzisa i-pipette.
4. Uqwalaselo kwimizuzu emi-5-10, alusebenzi emva kwemizuzu eli-15.
[Isiphumo isigwebo]
* Okuhle (+): Ubukho bebhanti ezibomvu zewayini kuzo zombini umgca wolawulo C kunye nomgca wobhaqo T ubonise ukuba isampuli iqulethe i-antibody yentsholongwane yehagu yehagu.
* Engalunganga (-): Akukho mbala uphuhliswayo kwi-T-reyi ubonisa ukuba isampulu ayinaso nasiphi na isilwa-mntu esichasene nentsholongwane ye-classical swine fever.
* Ayisebenzi: Akukho QC Line C okanye Whiteboard ekhoyo ebonisa inkqubo engalunganga okanye ikhadi elingasebenziyo.Nceda uphinde uvavanye.
[Ukulumkela]
I-1.Le mveliso yikhadi lokubona ngokukhawuleza kwi-vitro, ayikwazi ukuphinda isetyenziswe.
I-2.Le mveliso ivakalelwa kwiqondo lokushisa eligqithiseleyo, kungekhona iqhwa.
3.Le mveliso inovakalelo olugqithisileyo kumswakama, ukuba ayisebenzisi ngokukhawuleza, musa ukuyikrazula i-aluminium foil bag evulekileyo.Nceda uyisebenzise ngokukhawuleza emva kokukhulula, kwaye ayinakudlula iyure enye.
4. Nceda uqinisekise ukuba izixhobo zokuqokelela igazi ezisetyenziswa kwinkqubo yokuqokelela igazi zizixhobo zokuqokelela igazi ezilahlwayo.Ukuba isixhobo sokuqokelela igazi sisixhobo esiphinda sisetyenziswe ekuqokeleleni igazi, kufuneka sibulawe inzala phambi kokuba sisetyenziswe yihagu elandelayo.
5. Kufuneka udibane negazi lesilwanyana ekuvavanyeni, zama ukunxiba iiglavu zokusebenzisa, kwaye emva kovavanyo ngokuhambelana nemimiselo, uphathe ngokufanelekileyo iisampuli zegazi.kunye nezixhobo ezinxulumeneyo.
[Unyino lwesicelo]
Le mveliso yikiti yokuxilonga i-immunological, kwaye isetyenziselwa ukubonelela ngeziphumo zovavanyo olusemgangathweni ukubhaqwa kwezonyango kwizifo zezilwanyana.Ukuba kukho ukuthandabuza malunga neziphumo zovavanyo, nceda usebenzise ezinye iindlela zokuxilonga (i-PCR, uvavanyo lokuhlukaniswa kwe-pathogen, njl.) ukwenza uhlalutyo olongezelelweyo kunye nokuxilongwa kweesampuli ezifunyenweyo.Qhagamshelana nogqirha wezilwanyana wengingqi yakho malunga nohlalutyo lwezifo.
[Ugcino kunye nokuphelelwa]
Le mveliso kufuneka igcinwe ku-2 ℃–40℃ kwindawo epholileyo, eyomileyo kude nokukhanya kwaye ingakhenkcezi;Isebenza iinyanga ezingama-24.Jonga iphakheji yangaphandle yomhla wokuphelelwa kunye nenombolo yebhetshi.
