Intsholongwane kaGawulayo 1/2 kwiKhasethi yoVavanyo oluKhawulezayo
Ikhithi yoVavanyo oluKhawulezayo lwe-HIV Antibody
USHWANKATHELO
Indlela eqhelekileyo yokufumanisa usulelo olune-HIV kukuqwalasela ubukho be-antibodies kwintsholongwane ngendlela ye-EIA elandelwa kukuqinisekiswa nge-Western Blot.Uvavanyo lwe-HIV lwe-One Step luvavanyo olulula, olubonayo lomgangatho olufumanisa izilwa-buhlungu kwigazi Lonke lomntu/iserum/plasma.Uvavanyo lusekelwe kwi-immunochromatography kwaye lunokunika umphumo kwimizuzu eyi-15.
IIREGENTS KUNYE NEZINTO EZIBONISWAYO
Isixhobo sokuvavanya ifoyile nganye efakwe kwi-desiccant
- Test Cassette 25 pcs / ibhokisi
- Ingca yeplastiki elahlayo i-25 pcs / ibhokisi
- Buffer 1 pcs / ibhokisi
- Incwadi yomyalelo 1 iipcs / ibhokisi
IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA
Ulawulo olulungileyo nolungalunganga (lufumaneka njengento eyahlukileyo)
UKUGCINWA NOZINZI
Iikhithi zovavanyo kufuneka zigcinwe kwi-2-30℃kwipokhi evaliweyo naphantsi kweemeko ezomileyo.
UKUQOKELELWA KWEsampuli NOKUGCINWA
1) Qokelela iGazi Elipheleleyo / iSerum / iisampulu zePlasma ngokulandela iinkqubo eziqhelekileyo zelebhu yonyango.
I-2) Ukugcinwa: Igazi elipheleleyo alinakuba ngumkhenkce.Umzekelo kufuneka ufakwe efrijini ukuba awusetyenziswanga kwangolo suku lokuqokelela.Iisampulu kufuneka zifakwe emkhenkceni ukuba azisetyenziswanga kwiintsuku ezi-3 zokuqokelelwa.Kuphephe ukukhenkceza kunye nokunyibilika iisampuli ngaphezu kwamaxesha angama-2-3 ngaphambi kokuba usebenzise.I-0.1% ye-Sodium Azide inokongezwa kwi-specimen njenge-preservative ngaphandle kokuchaphazela iziphumo zovavanyo.
INKQUBO YOVAVANYO
1) Ukusebenzisa i-dropper yeplastiki evalelweyo kwisampulu, khupha i-1 drop (10μl) yeGazi eliPheleleyo / iSerum / iPlasma kwisampulu esetyhula kwiqula lekhadi lovavanyo.
2) Yongeza ama-2 amaconsi eSample Diluent kwisampulu kakuhle, ngokukhawuleza emva kokuba i-specimen yongezwe, ukusuka kwi-dropper tip diluent vial (okanye yonke imixholo evela kwi-ampule yovavanyo olulodwa).
3) Tolika iziphumo zovavanyo kwimizuzu eyi-15.
UKUFUNDA IZIPHUMO ZOMVAVANYO
1)Okuhle: Zombini ibhendi yovavanyo ebomvu ebomvu kunye nebhendi yolawulo ebomvu ebomvu ibonakala kwinwebu.Okukhona isezantsi ingxinano ye-antibody, kokukhona iba buthathaka ibhendi yovavanyo.
2) Ibi: Kuphela ibhendi yolawulo ebomvu ebomvu ibonakala kwi-membrane.Ukungabikho kwebhendi yovavanyo kubonisa umphumo ombi.
3)Isiphumo esingasebenziyo:Kufuneka kuhlala kukho ibhendi yokulawula ebomvu ebomvu kwindawo yolawulo, nokuba sithini na isiphumo sovavanyo.Ukuba ibhendi yolawulo ayibonwa, uvavanyo luthathwa njengento engavumelekanga.Phinda uvavanyo usebenzisa isixhobo sovavanyo esitsha.
Qaphela: Kuyinto eqhelekileyo ukuba nebhendi yolawulo ekhanyiswe kancinane eneisampulu ezomeleleyo eziqinisekileyo, ukuba nje ibonakala ngokucacileyo.
UMDA
I-1) Kuphela ecacileyo, esitsha, ekhululekileyo iGazi elipheleleyo / iSerum / iPlasma enokusetyenziswa kolu vavanyo.
2) Iisampulu ezintsha zezona zilungileyo kodwa iisampulu ezikhenkcezisiweyo zingasetyenziswa.Ukuba isampuli ikhenkcezisiwe, kufuneka ivunyelwe ukuba inyibilike kwindawo ethe nkqo kwaye ikhangelwe ukuba ayinamanzi na.IGazi Elipheleleyo alinakukhenkcezwa.
3) Musa ukuphazamisa isampuli.Faka i-pipette ngaphantsi komphezulu wesampuli ukuqokelela i-Specimen.
