(TP ) Ikhasethi yeSyphilis Blood Rapid Test
Ukufumanisa kwangethuba i-TP Syphilis Blood Test Kit Cassette
USHWANKATHELO
Indlela eqhelekileyo yokubona usulelo nge-TP isetyenziselwa ukufunyaniswa kwe-in vitro qualitative ye-Syphilis (TP) Antibody kwigazi elipheleleyo lomntu, i-serum okanye iisampulu ze-plasma. Uvavanyo lusekelweIndlela yegolide yeColloidalkwaye inokunika isiphumo kwimizuzu eli-15.
UKUSETYENZISWA OKUHLOSIWEYO
UVavanyo lweSinyathelo esinye seTP yiColloidal Gold eyongeziweyo,.Ngokonyango, le mveliso isetyenziselwa ukuxilongwa kwe-Treponema pallidum encedisayo.Le mveliso isetyenziselwa abasebenzi bezonyango kuphela.
UMGAQO WENKQUBO
Imveliso ithatha umgaqo wesangweji ye-antigen kabini.Xa isampuli iqulethe i-antibody ye-Treponema pallidum, i-antibody ye-Treponema pallidum kwisampulu iphendula kunye negolide ye-colloidal ebhalwe-syphilis recombinant antigen 1 kwi-pad yokubopha ukwenza i-antigen-antibody complex.I-complex i-chromato-graphed phambili nge-capillary action kwaye ibanjwe yi-syphilis antigen 2 ehlanganiswe kwindawo ye-detecticn (umgca we-T) we-nitrocellulose membrane, kunye nebhendi ebomvu ibonakala.Ikhompleksi iyaqhubeka ukunyuswa ngekhromatograph, kwaye inkukhu i-lgY colloidal gold marker ibanjwa yi-anti-chicken lgY antibody yebhokhwe egqunywe kwindawo yolawulo lomgangatho (umgca C) we-nitrocellulose inwebu, kwaye kuvela ibhande elibomvu.Xa umxholo wohlalutyi kwisampulu ungaphantsi kunomlinganiselo omncinci wokufumanisa, indawo yokufumanisa (i-T line) ayiphuhlisi umbala.
ICANDELO ELIPHAMBILI
1.Iphedi yovavanyo, nganye ipakishwe kwibhegi yefoyile ye-aluminium (1piece/bag,1/5/10/25/50iqhekeza/ikhithi
2.Ingca yeplastiki elahlayo (1picce/ibhegi,1/5/10/25/50 iqhekeza/ikhithi)
3.Ingxowa yenkunkuma yezonyango(1picce/bag,1/5/10/25/50 piece(s)/kit)
4. Incwadi yemiyalelo (1ikopi/ibhegi, ikopi eyi-1/ikhithi)
Qaphela: Amalungu kwiikhithi zamanani eebhetshi ahlukeneyo akatshintshi.
AMANQAKU OZIKHETHA
Isampulu yokuxutywa kwesampulu (iqhekeza/ibhegi,1/5/10/25/50(s)/ikhithi)
Iphedi yomqhaphu yotywala(iqhekeza/ibhegi,1/5/10/25/50 i(s)/ikhithi)
口 Inaliti yokuqokelela igazi(iqhekeza/ibhegi,1/5/10/25/50(s)/ikhithi)
IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA
Ulawulo olulungileyo nolungalunganga (lufumaneka njengento eyahlukileyo)
UKUGCINWA NOZINZI
Ipakethe yokuqala kufuneka igcinwe kwindawo eyomileyo kwi-4-30 ℃ ikhuselwe ekukhanyeni, kwaye ungakhenkcezi.
IZILUMKISO NEMIGAQO
Ikhithi izinzile ngaphakathi komhla wokuphelelwa oshicilelwe kwilebhile.Iphedi yovavanyo kufuneka isetyenziswe kwiyure ye-l phantsi kwemeko ye-4-30℃ kunye nokufuma <65% emva kokuba ibhegi yefoyile ye-aluminiyam ingapakishwa.Kunconywa ukuba uyisebenzise ngokukhawuleza phantsi kweqondo lokushisa eliphezulu okanye iimeko zomswakama ophezulu.
UKUQOKELELWA KWEsampuli NOKUGCINWA
2. Ukugcinwa kwesampuli
2.1 Igazi elipheleleyo;Iityhubhu ze-Anticoagulant zisetyenziselwa ukuqokelela igazi, kwaye ziqhelekileyoi-anticoagulants ingasetyenziswa;ukuba iisampulu zegazi elipheleleyo azinakusetyenziswa ngokukhawuleza emva kokoingqokelela, zinokugcinwa kwi-2-8 ° C iintsuku ezi-3, kwaye iisampuli azikwazi ukukhenkcezwa.
2.2 I-Serum / i-plasma: Isampuli ingagcinwa kwi-2-8 ℃ iintsuku ze-7, kwaye kufuneka ibeigcinwe kwi -20 ℃ yokugcina ixesha elide.
3.Kufuneka kusetyenziswe iisampulu ezingekho hemolyzed kuphela.Iisampulu zehemolyzed kakhulu kufuneka zisetyenziswe.mazenziwe imizekelo.
4 Iisampulu ezifakwe efrijini kufuneka zibuyiselwe kubushushu begumbi phambi kovavanyo.Iiisampulu ezikhenkcezisiweyo kufuneka zinyibilike ngokupheleleyo, zifudunyezwe, kwaye zixutywe ngokulinganayo ngaphambilisebenzisa.Musa ukuba ngumkhenkce kwaye unyibilike ngokuphindaphindiweyo
INKQUBO YOVAVANYO
1) Ukusebenzisa i-dropper yeplastiki evalelweyo kwisampulu, khupha i-1 drop (10μl) yeGazi eliPheleleyo / iSerum / iPlasma kwisampulu esetyhula kwiqula lekhadi lovavanyo.
2) Yongeza ama-2 amaconsi eSample Diluent kwisampulu kakuhle, ngokukhawuleza emva kokuba i-specimen yongezwe, ukusuka kwi-dropper tip diluent vial (okanye yonke imixholo evela kwi-ampule yovavanyo olulodwa).
3) Tolika iziphumo zovavanyo kwimizuzu eyi-15.
Amanqaku:
1) Ukusebenzisa isixa esaneleyo se-diluent yesampulu kubalulekile kwisiphumo sovavanyo esisebenzayo.Ukuba ukufuduka (ukumanzisa kwe-membrane) akubonwa kwifestile yovavanyo emva komzuzu omnye, yongeza enye ithontsi le-diluent kwisampulu kakuhle.
I-2) Iziphumo ezilungileyo zingavela ngokukhawuleza nje ngomzuzu omnye wesampuli kunye namanqanaba aphezulu e-TP antibodies.
3) Musa ukutolika iziphumo emva kwemizuzu engama-20
UKUFUNDA IZIPHUMO ZOMVAVANYO
1)Okuhle: Zombini ibhendi yovavanyo ebomvu ebomvu kunye nebhendi yolawulo ebomvu ebomvu ibonakala kwinwebu.Okukhona isezantsi ingxinano ye-antibody, kokukhona iba buthathaka ibhendi yovavanyo.
2) Ibi: Kuphela ibhendi yolawulo ebomvu ebomvu ibonakala kwi-membrane.Ukungabikho kwebhendi yovavanyo kubonisa umphumo ombi.
3)Isiphumo esingasebenziyo:Kufuneka kuhlala kukho ibhendi yokulawula ebomvu ebomvu kwindawo yolawulo, nokuba sithini na isiphumo sovavanyo.Ukuba ibhendi yolawulo ayibonwa, uvavanyo luthathwa njengento engafanelekanga.Phinda uvavanyo usebenzisa isixhobo sovavanyo esitsha.
Qaphela: Kuyinto eqhelekileyo ukuba nebhendi yolawulo ekhanyiswe kancinane eneisampulu ezomeleleyo eziqinisekileyo, ukuba nje ibonakala ngokucacileyo.
UMDA
I-1) Kuphela ecacileyo, esitsha, ekhululekileyo iGazi elipheleleyo / iSerum / iPlasma enokusetyenziswa kolu vavanyo.
2) Iisampulu ezintsha zezona zilungileyo kodwa iisampulu ezikhenkcezisiweyo zingasetyenziswa.Ukuba isampuli ikhenkcezisiwe, kufuneka ivunyelwe ukuba inyibilike kwindawo ethe nkqo kwaye ikhangelwe ukuba ayinamanzi na.IGazi Elipheleleyo alinakukhenkcezwa.
3) Musa ukuphazamisa isampuli.Faka i-pipette ngaphantsi komphezulu wesampuli ukuqokelela i-Specimen.
