Inyathelo elinye lovavanyo lwe-HCV Cassette(Igazi Elipheleleyo/iSerum/Plasma)
UVAVANYO LWE-HCV INYATHELO ELINYE (igazi elipheleleyo/iSerum/i-Plasma)
USHWANKATHELO
Indlela eqhelekileyo yokubona usulelo nge-HCV kukuqwalasela ubukho be-antibodies kwintsholongwane ngendlela ye-EIA elandelwa kukuqinisekiswa nge-Western Blot.Uvavanyo lwe-One Step HCV luvavanyo olulula, olubonakalayo olubona izilwa-buhlungu kwiGazi Lonke lomntu/iserum/iplasma.Uvavanyo lusekelwe kwi-immunochromatography kwaye lunokunika umphumo kwimizuzu eyi-15.
UKUSETYENZISWA OKUHLOSIWEYO
INyathelo elinye loVavanyo lwe-HCV yiColloidal Gold ephuculweyo, i-Immunochromatoraphic Assay ekhawulezayo yokubona umgangatho we-antibodies kwi-Hepatitis C Virus (HCV) kwiGazi Loluntu Lonke / iSerum / Plasma.Olu vavanyo luvavanyo lwe-screening kwaye zonke ii-positives kufuneka ziqinisekiswe ngokusebenzisa uvavanyo olulolunye olufana ne-Western Blot.Uvavanyo lwenzelwe ukusetyenziswa koBuchule bezeMpilo kuphela.Zombini uvavanyo kunye neziphumo zovavanyo zenzelwe ukuba zisetyenziswe ngabasebenzi bezonyango kunye nezomthetho kuphela, ngaphandle kokuba kugunyaziswe ngummiselo kwilizwe elisetyenziswayo.Uvavanyo akufanele lusetyenziswe ngaphandle kweliso elifanelekileyo.
UMGAQO WENKQUBO
Uvavanyo luqala ngesampulu esetyenziswa kwisampulu kakuhle kunye nokongezwa kwesampulu enikeziweyo yediluent kwangoko.I-HCV i-antigen-Colloidal Gold conjugate efakwe kwi-pad yesampuli isabela kunye ne-antibody ye-HCV ekhoyo kwi-serum okanye kwi-plasma, yenze i-conjugate/HCV antibody complex.Njengoko umxube uvunyelwe ukuba ufuduke ecaleni komtya wovavanyo, i-conjugate/HCV antibody complex ibanjwa yiprotein ebopha i-antibody-binding A immobilized kwi-membrane eyenza ibhanti enemibala kwindawo yovavanyo.Isampuli engalunganga ayivelisi umgca wokuvavanya ngenxa yokungabikho kwe-Colloidal Gold conjugate / HCV antibody complex.Ii-antigens ezisetyenzisiweyo kuvavanyo ziiprotheni eziphinda ziphinde zihlangane ezihambelana nemimandla engasebenzi kakhulu ye-HCV.Ibhendi yokulawula enemibala kwindawo yokulawula ibonakala ekupheleni kwenkqubo yokuvavanya kungakhathaliseki ukuba yintoni umphumo wokuvavanya.Eli qela lolawulo lisisiphumo se-Colloidal Gold conjugate ebophelelayo kwi-anti-HCV antibody immobilized kwi-membrane.Umgca wokulawula ubonisa ukuba i-conjugate ye-Colloidal Gold iyasebenza.Ukungabikho kwebhendi yolawulo kubonisa ukuba uvavanyo aluvumelekanga.
IIREGENTS KUNYE NEZINTO EZIBONISWAYO
Isixhobo sokuvavanya ifoyile nganye efakwe kwi-desiccant
• Idropha yeplastiki.
• Isampulu yoDiluyinti
• ipakethe Faka
IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA
Ulawulo olulungileyo nolungalunganga (lufumaneka njengento eyahlukileyo)
UKUGCINWA NOZINZI
Iikhithi zovavanyo kufuneka zigcinwe kwi-2-30℃kwipokhi evaliweyo naphantsi kweemeko ezomileyo.
IZILUMKISO NEMIGAQO
1) Zonke iziphumo ezilungileyo kufuneka ziqinisekiswe ngenye indlela.
2) Phatha yonke imizekelo ngokungathi inokosulela.Nxiba iiglavu kunye nempahla yokukhusela xa uphethe imizekelo.
I-3) Izixhobo ezisetyenziselwa ukuvavanya kufuneka zifakwe kwi-autoclaved ngaphambi kokulahlwa.
4) Musa ukusebenzisa izixhobo zekiti ngaphaya kwemihla yazo yokuphelelwa yisikhathi.
5) Musa ukutshintshanisa ii-reagents ezivela kwiindawo ezahlukeneyo.
UKUQOKELELWA KWEsampuli NOKUGCINWA
1) Qokelela igazi elipheleleyo / iSerum / iisampulu zePlasma ngokulandela iinkqubo zarhoqo zelebhu yonyango.
I-2) Ukugcinwa: Igazi elipheleleyo alinakuba ngumkhenkce.Umzekelo kufuneka ufakwe efrijini ukuba awusetyenziswanga kwangolo suku lokuqokelela.Iisampulu kufuneka zifakwe emkhenkceni ukuba azisetyenziswanga kwiintsuku ezi-3 zokuqokelelwa.Kuphephe ukukhenkceza kunye nokunyibilika iisampulu amaxesha angaphezu kwama-2-3 phambi kokusetyenziswa.I-0.1% ye-Sodium Azide inokongezwa kwi-specimen njenge-preservative ngaphandle kokuchaphazela iziphumo zovavanyo.
INKQUBO YOVAVANYO
1) Ukusebenzisa i-dropper yeplastiki evalelweyo kwisampulu, khupha i-1 drop (10μl) yeGazi eliPheleleyo / iSerum / iPlasma kwisampulu esetyhula kwiqula lekhadi lovavanyo.
2) Yongeza ama-2 amaconsi eSample Diluent kwisampulu kakuhle, ngokukhawuleza emva kokuba i-specimen yongezwe, ukusuka kwi-dropper tip diluent vial (okanye yonke imixholo evela kwi-ampule yovavanyo olulodwa).
3) Tolika iziphumo zovavanyo kwimizuzu eyi-15.
Amanqaku:
1) Ukusebenzisa isixa esaneleyo se-diluent yesampulu kubalulekile kwisiphumo sovavanyo esisebenzayo.Ukuba ukufuduka (ukumanzisa kwe-membrane) akubonwa kwifestile yovavanyo emva komzuzu omnye, yongeza enye ithontsi le-diluent kwisampulu kakuhle.
2) Iziphumo ezilungileyo zinokuvela ngokukhawuleza nje ngomzuzu omnye kwisampulu enamanqanaba aphezulu e-HCV antibodies.
3) Musa ukutolika iziphumo emva kwemizuzu engama-20
UKUFUNDA IZIPHUMO ZOMVAVANYO
1)Okuhle: Zombini ibhendi yovavanyo ebomvu ebomvu kunye nebhendi yolawulo ebomvu ebomvu ibonakala kwinwebu.Okukhona isezantsi ingxinano ye-antibody, kokukhona iba buthathaka ibhendi yovavanyo.
2) Ibi: Kuphela ibhendi yolawulo ebomvu ebomvu ibonakala kwi-membrane.Ukungabikho kwebhendi yovavanyo kubonisa umphumo ombi.
3)Isiphumo esingasebenziyo:Kufuneka kuhlala kukho ibhendi yokulawula ebomvu ebomvu kwindawo yolawulo, nokuba sithini na isiphumo sovavanyo.Ukuba ibhendi yolawulo ayibonwa, uvavanyo luthathwa njengento engafanelekanga.Phinda uvavanyo usebenzisa isixhobo sovavanyo esitsha.
Qaphela: Kuyinto eqhelekileyo ukuba nebhendi yolawulo ekhanyiswe kancinane eneisampulu ezomeleleyo eziqinisekileyo, ukuba nje ibonakala ngokucacileyo.
UMDA
I-1) Kuphela ecacileyo, esitsha, ekhululekileyo iGazi elipheleleyo / iSerum / iPlasma enokusetyenziswa kolu vavanyo.
2) Iisampulu ezintsha zezona zilungileyo kodwa iisampulu ezikhenkcezisiweyo zingasetyenziswa.Ukuba isampuli ikhenkcezisiwe, kufuneka ivunyelwe ukuba inyibilike kwindawo ethe nkqo kwaye ikhangelwe ukuba ayinamanzi na.IGazi Elipheleleyo alinakukhenkcezwa.
3) Musa ukuphazamisa isampuli.Faka i-pipette ngaphantsi komphezulu wesampuli ukuqokelela i-Specimen.
