Ukusukela oko kwaqala ubhubhane, uvavanyo loxilongo ludlale indima ebalulekileyo ekulawuleni ukusasazeka kwe-SARS-CoV-2, intsholongwane ebangelaI-COVID 19.Iimvavanyo ze-antigen ezikhawulezayoeyenziwa ekhaya okanye kwimeko yeklinikhi inika iziphumo kwimizuzu eyi-15 okanye ngaphantsi.Okukhona umntu efunyanwe kwangethuba, kokukhona enokufuna unyango kwaye azimele yedwa kwabanye.Kodwa xa iintlobo ezintsha zentsholongwane zivela, ezo zahlulo zinokungabonwa kolu vavanyo.
Uninzi lovavanyo olukhawulezayo lwe-antigen lwenzelwe ukufumanisa i-SARS-CoV-2 nucleocapsid protein okanye i-N-protein.Le proteni ifumaneka ngobuninzi kwiintsholongwane zentsholongwane kunye nabantu abosulelekileyo.Ikhithi yovavanyo olukhawulezayoIdla ngokuba nezilwa-buhlungu ezimbini ezahlukeneyo ezibophelela kwiindawo ezahlukeneyo zeprotheni ye-N.Xa i-antibody ibophelela kwiprotheni ye-N kwisampulu, umgca onemibala okanye olunye uphawu luvela kwikhithi yovavanyo, ebonisa usulelo.
Iprotheyini N iqulathe 419 amino acid iiyunithi isakhiwo.Nayiphi na kuzo inokutshintshwa yenye i-amino acid ngokuguqulwa kwenguqu.Iqela lophando elikhokelwa nguPh.D.UFilipu Frank kunye no-Eric Ortlund beYunivesithi yase-Emory bazimisele ukuphanda indlela olu tshintsho olulodwa lwe-amino acid oluchaphazela ngayo ukusebenza kovavanyo olukhawulezayo lwe-antigen.Basebenzise ubuchule obubizwa ngokuba sisikena esinzulu soguqulo ukuvavanya ngaxeshanye ukuba uguqulo ngalunye lweprotein ye-N yentsholongwane luchaphazela njani ukubophelela kwi-antibody yoxilongo.Iziphumo zabo zapapashwa kwiCell ngoSeptemba 15, 2022.
Abaphandi benza ilayibrari ebanzi malunga neenguqu zeprotheyini ze-8,000 N.Ezi zantlukwano zenza ngaphezu kwe-99.5% yazo zonke iinguqu ezinokwenzeka.Baye bavavanya indlela eyahlukileyo nganye enxibelelana ngayo kunye ne-17 ezahlukeneyo zokuxilonga ezisetyenziswa kwiimvavanyo ezili-11 ezifumanekayo kurhwebo olukhawulezayo lwe-antigen, kubandakanya nesiqhelo.izixhobo zasekhaya.
Iqela livavanye ukuba zeziphi iinguqu ze-N-protein ezichaphazela ukuqatshelwa kwe-antibody.Ngokusekwe kolu lwazi, benze "iprofayili yokuguqula i-escape" ye-antibody nganye yokuxilonga.Le nkcazo ichaza utshintsho oluthile kwiprotheni ye-N enokuthi ichaphazele amandla e-antibody ukuba abophe ekujoliswe kuyo.Uhlalutyo lubonise ukuba ii-antibodies ezisetyenziswa kuvavanyo lwanamhlanje olukhawulezayo ziyaqaphela kwaye zibophe zonke iiyantlukwano zangaphambili nezangoku ze-SARS-CoV-2 zenkxalabo kunye nenkxalabo.
Nangona izilwa-buhlungu ezininzi zoxilongo zibona ummandla omnye weprotheyini ye-N, abaphandi bafumanise ukuba i-antibody nganye inophawu olulodwa lotshintsho lokubaleka.Njengoko intsholongwane ye-SARS-CoV-2 iqhubeka nokutshintsha kwaye ivelisa izinto ezintsha ezahlukeneyo, le datha inokusetyenziselwa ukufola ii-antibodies zekiti ezinokufuna ukuphinda zihlolwe.
"Ukuchongwa ngokuchanekileyo nangokufanelekileyo kwabantu abosulelekileyo kuhlala kusisicwangciso esibalulekileyo sokunciphisa i-COVID-19, kwaye isifundo sethu sibonelela ngolwazi malunga notshintsho lwe-SARS-CoV-2 lwexesha elizayo olunokuphazamisana nokubhaqwa," utshilo u-Ortlund."Iziphumo ezichazwe apha zisivumela ukuba siqhelane ngokukhawuleza nale ntsholongwane njengoko iintlobo ezintsha ziqhubeka zivela, zibonisa iziphumo ezikhawulezileyo zempilo yoluntu."
Imvelaphi: I-Mutation Deep Scan ibona utshintsho lokubaleka kwi-SARS-CoV-2 nucleocapsid isebenzisa iimvavanyo ze-antigen ezifumanekayo ngoku.Frank F., Kin MM, Rao A., Bassit L., Liu H, Bowers HB, Patel AB, Kato ML, Sullivan JA, Greenleaf M., Piantadosi A., Lam VA, Hudson VH, Ortlund EA cell.2022 Septemba 15; 185 (19): 3603-3616.e13.UMphathiswa Wezangaphakathi: 10.1016/j.cell.2022.08.010.Agasti 29, 2022 PMID: 36084631.
Inkxaso-mali: Iziko leSizwe le-Biomedical Imaging kunye ne-Bioengineering NIH (NIBIB), i-National Institute of Diabetes, i-Digestive and Kidney Diseases (NIDDK) kunye ne-National Institute of Allergy and Infectious Diseases (NIAID), i-American Heart Association.
I-NIH yoPhando lweMiba luhlaziyo lweveki lweziphumo eziphambili zophando lwe-NIH ezihlaziywe ziingcali ze-NIH.Ipapashwe yi-Ofisi yoNxibelelwano kunye neMicimbi yoLuntu yoMlawuli weZiko lezeMpilo leSizwe.