[OKUHLOSIWEYO UKUSETYENZISWA]

UVavanyo oluKhawulezayo loMkhuhlane A+B luvavanyo olukhawulezayo olubonwayo lomgangatho, ukubhaqwa okucingelwayo komkhuhlane A kunye no-B ii-antigens zentsholongwane egazini zenza umqala kunye neesampulu ze-nasopharyngeal swab.Uvavanyo lwenzelwe ukusetyenziswa njengoncedo ekuxilongeni okukhawulezileyo ukwahluka kohlobo lomkhuhlane obukhali A kunye nohlobo lwentsholongwane ye-B.

UMGAQO

Ikhasethi yoVavanyo oluKhawulezayo loMkhuhlane A+B ibhaqa ii-antigens zentsholongwane yomkhuhlane A kunye no-B ngokutolika okubonakalayo kophuhliso lombala kumgca.Izilwa-buhlungu ezichasene nomkhuhlane A kunye no-B azishukumi kwindawo yovavanyo A kunye no-B wenwebu ngokulandelelanayo.Ngexesha lovavanyo, umfuziselo okhutshiweyo udibana nezilwa-ntsholongwane ezilwa nomkhuhlane A kunye no-B ezidityaniswe kumasuntswana anemibala kwaye zifakwe ngaphambili kwisampulu yovavanyo.Umxube ke ufuduka kwi-membrane ngesenzo se-capillary kwaye udibanise ne-reagents kwi-membrane.Ukuba kukho ii-antigens zentsholongwane zomkhuhlane A kunye no-B ezaneleyo kumzekelo, i(s) ezinemibala ziyakwenzeka kwindawo yovavanyo yenwebu.Ubukho bebhanti enemibala kwingingqi ye-A kunye / okanye ye-B ibonisa umphumo ochanekileyo we-antigens ethile yentsholongwane, ngelixa ukungabikho kwayo kubonisa umphumo ombi.Ukubonakala kwebhanti enemibala kwindawo yokulawula isebenza njengolawulo lwenkqubo, ebonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.

UKUGCINWA NOZINZI

1.Ikhithi kufuneka igcinwe kwi-2-30°C de kube ngumhla wokuphelelwa kwexesha uprintwe kwisingxobo esivaliweyo.

2. Uvavanyo maluhlale kwisingxobo esitywiniweyo de lusetyenziswe.

3.Musa ukuba ngumkhenkce.

4.Inkathalo kufuneka ithathwe ukukhusela amacandelo ekhithi ekungcoleni.Musa ukusebenzisa ukuba kukho ubungqina bokungcoliseka kwe-microbial okanye imvula.Ungcoliseko lwebhayoloji lwezixhobo zokukhupha, izikhongozeli okanye iirejenti kunokukhokelela kwiziphumo ezingezizo.

INKQUBO

Zisa iimvavanyo, imizekelo, kunye/okanye nolawulo kwiqondo lobushushu legumbi (15-30°C) phambi kokusetyenziswa.

1.Susa uvavanyo kwingxowa yalo evaliweyo, kwaye uyibeke kwindawo ecocekileyo, esemgangathweni.Bhala iKhasethi ngesazisi sesigulane okanye solawulo.Ukuze ufumane iziphumo ezingcono, uvavanyo kufuneka lwenziwe kwiyure enye.

2. Gently mix Extraction reagent solution.Yongeza amaconsi ama-6 eSisombululo sokukhupha kwi-tube yokukhupha.

3.Beka isampuli ye-swab yesigulane kwi-Extraction Tube.Roll swab ubuncinane amaxesha ali-10 ngelixa ucinezela i-swab ngezantsi kunye necala le-Extraction Tube.Qeqesha intloko ye-swab ngaphakathi kwi-Extraction Tube xa uyisusa.Zama ukukhulula ulwelo oluninzi kangangoko.Lahla i-swab esetyenzisiweyo ngokuhambelana neprothokholi yakho yokulahla inkunkuma ye-biohazard.

4.Beka kwi-tip ye-tube, uze udibanise i-4 yehla yesampuli ekhutshwe kwisampuli kakuhle.Musa ukuyiphatha okanye uyishukumise iCassette yoVavanyo de uvavanyo lugqitywe kwaye lulungele ukufundwa.

5.Njengoko uvavanyo luqala ukusebenza, umbala uya kufuduka kwi-membrane.Lindela ukuba i(ama)bhanti enemibala ivele.Isiphumo kufuneka sifundwe ngemizuzu eyi-10.Musa ukutolika umphumo emva kwemizuzu engama-20.

UKUTOLIKWA KWEZIPHUMO

Vumela ikhasethi yovavanyo kunye nemizekelo ukuba ilingane nobushushu (15-30 ℃ okanye 59-86℉) phambi kovavanyo.

1. Susa ikhasethi yovavanyo kwisingxobo esivaliweyo.

2. Ukubuyisela umva ityhubhu yokutsalwa komzekelo, Ukubamba utsalo lomfanekiso

ityhubhu ime nkqo, tshintshela amathontsi ama-3 (malunga ne-100μl) kumzekelo

kakuhle(I) yekhasethi yovavanyo, emva koko uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

Linda imigca enemibala ivele.Ukutolika iziphumo zovavanyo kwimizuzu eyi-15.Sukuzifunda iziphumo emva kwemizuzu engama-20.

IMIDA YOVAVANYO

1.Ikhasethi yoVavanyo oluKhawulezayo lweFlu A+B yeyokusetyenziswa koxilongo lwe-in vitro yobungcali, kwaye kufuneka isetyenziswe kuphela ukufumanisa umgangatho womkhuhlane A kunye/okanye u-B.

2.I-etiology yosulelo lokuphefumula olubangelwa yi-microorganisms ngaphandle kwe-influenza A okanye i-virus ye-B ayiyi kusekwa ngolu vavanyo.Ikhasethi yoVavanyo lweFlu A+B oluKhawulezayo iyakwazi ukubona omabini amasuntswana omkhuhlane osebenzayo kunye nongasebenziyo.Ukusebenza kwe-Flu A+B Rapid Test Cassette ixhomekeke kumthwalo we-antigen kwaye ayinakho ukuhambelana nenkcubeko yeseli eyenziwa kwi-specimen efanayo.

3.Ukuba isiphumo sovavanyo sibi kwaye iimpawu zeklinikhi ziqhubeka, uvavanyo olongezelelweyo usebenzisa ezinye iindlela zeklinikhi ziyacetyiswa.Iziphumo ezibi azenzi nangaliphi na ixesha ubukho bomkhuhlane A kunye/okanye i-antigens yentsholongwane ye-B kumzekelo, njengoko zisenokubakho ngaphantsi komgangatho ophantsi wovavanyo.Njengazo zonke iimvavanyo zokuxilonga, ukuxilongwa okuqinisekisiweyo kufuneka kwenziwe kuphela ngugqirha emva kokuba zonke iziphumo zeklinikhi kunye nelebhu ziye zavavanywa.

4.Ubunyani bekhasethi yoVavanyo oluKhawulezayo lweFlu A+B abuqinisekiswanga ukuze kuchongwe okanye kungqinwe ii-cell culture zodwa.

5. Ukungonelanga okanye ukungafanelekanga komfanekiso wokuqokelelwa, ukugcinwa, kunye nokuthuthwa kunokuvelisa iziphumo zovavanyo olungeyonyani.

6.Nangona olu vavanyo lubonisiwe ukuba lubhaqa iintsholongwane zomkhuhlane weentaka ezikhulisiwe, kuquka intsholongwane yomkhuhlane weentaka A subtype H5N1, iimpawu zokusebenza kolu vavanyo kunye nemizekelo yabantu abosulelwe yi-H5N1 okanye ezinye iintsholongwane zomkhuhlane weentaka azaziwa.

7.Iimpawu zokusebenza zomkhuhlane A zasekwa xa umkhuhlane A/H3 kunye ne-A/H1 yayizezona ntsholongwane ziphambili zomkhuhlane A ezijikelezayo.Xa kuvela ezinye iintsholongwane zomkhuhlane A, iimpawu zokusebenza zinokwahluka.

8.Abantwana bathanda ukuphalaza intsholongwane ixesha elide kunabantu abadala, nto leyo enokubangela umahluko phakathi kwabantu abadala nabantwana.

9.Amaxabiso aqikelelwayo alungileyo nangalunganga axhomekeke kakhulu kuxhaphaka.Iziphumo zovavanyo ezingeyonyani zinokwenzeka kakhulu ngamaxesha omkhuhlane ophantsi xa ubukho jikelele buphakathi ukuya phantsi.

PHAWULA:

1.Ubunzima bombala kwindawo yokuvavanya (A / B) inokuhluka ngokuxhomekeka kugxininiso lohlalutyo olukhoyo kwi-specimen.Ngoko ke, nawuphi na umthunzi wombala kummandla wokuvavanya (A / B) kufuneka uthathwe njengento enhle.Nceda uqaphele ukuba olu luvavanyo lomgangatho kuphela, kwaye alukwazi ukumisela ugxininiso lwabahlalutyi kumzekelo.

I-2.I-specimen enganelanga umthamo, inkqubo yokusebenza engalunganga okanye iimvavanyo eziphelelwe lixesha zizona zizathu ezinokuthi zibangele ukungaphumeleli kwebhendi yolawulo.