I-COVID-19 Antigen Rapid Test Cassette ( Amathe )
Ikhasethi yoVavanyo oluKhawulezayo lwe-COVID-19 (i-Colloidal Gold) luvavanyo olukhawulezayo lwe-chromatographic immunoassay yokufunyaniswa komgangatho wokungathathi hlangothi kwezilwa-buhlungu ze-COVID-19 kwigazi lomntu lonke, i-serum, okanye iplasma njengoncedo ekufunyanisweni kobukho bezithinteli-zifo. kwi-COVID-19.
Inoveli coronaviruses yeyodidi lwe-β.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla.Ngokuqhelekileyo abantu basengozini.Okwangoku, izigulana ezosulelwe yinoveli coronavirus ngoyena mthombo wosulelo;abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela yi-1 ukuya kwiintsuku ze-14, ubukhulu becala iintsuku ezi-3 ukuya kwezi-7.Iimpawu eziphambili ziquka umkhuhlane, ukukhathala kunye nokukhwehlela okomileyo.Ukuxinana kweempumlo, iimpumlo, umqala obuhlungu, i-myalgia kunye ne-diarrhea zifumaneka kwiimeko ezimbalwa.
I-COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) luvavanyo olukhawulezayo olusebenzisa indibaniselwano yamasuntswana anemibala e-antigen e-S-RBD ukuze kubonwe izilwa-buhlungu ezingathathi hlangothi kwi-COVID-19 kwigazi elipheleleyo lomntu, i-serum, okanye iplasma.
I-COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) yimwebu esemgangathweni esekwe kuvavanyo lokuqinisekisa ukuba kukho amajoni omzimba angasebenziyo kwi-COVID-19 egazini, kwiserum okanye kwiplasma.I-membrane ifakwe ngaphambili kunye ne-Angiotensin I-Converting Enzyme 2 (ACE2) kwindawo yovavanyo lomgca womgca.Ngexesha lovavanyo, igazi elipheleleyo, i-serum okanye i-plasma specimen idibana ne-S-RBD edityanisiweyo yegolide ye-colloid.Umxube ufudukela phezulu kwi-membrane ye-chromatographically ngesenzo se-capillary ukusabela nge-ACE2 kwi-membrane kwaye ivelise umgca onemibala.Ubukho balo mgca wemibala bubonisa umphumo ombi, ngelixa ukungabikho kwawo kubonisa umphumo omuhle.Ukuze usebenze njengolawulo lwenkqubo, umgca onemibala uya kuhlala utshintsha ukusuka kwiBlue ukuya kuBomvu kwindawo yokulawula umgca, ebonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.
| Izixhobo zovavanyo ezipakishwe ngumntu ngamnye | Isixhobo ngasinye siqulethe umtya onemibala edibanisayo kunye nezixhobo ezisebenzayo ezisasazeke ngaphambili kwimimandla ehambelanayo. |
| Iipayipi ezilahlayo | Ukongeza imizekelo sebenzisa |
| Isithinteli | I-phosphate buffered saline kunye nepreservative |
| Faka iphakheji | Ngomyalelo wokusebenza |
Izixhobo ezibonelelweyo
| ●Vavanya izixhobo | ●Iidropha |
| ● Isithinteli | ●Fakela ipakethe |
Izinto Eziyimfuneko Kodwa Ezingabonelelwanga
| ● Izikhongozeli zengqokelela yomfanekiso | ● Isibali-xesha |
| ●Iziko |
1. Ukusetyenziswa koxilongo lwe-in vitro kuphela.
2. Ungasebenzisi emva komhla wokuphelelwa yisikhathi oboniswe kwiphakheji.Musa ukusebenzisa uvavanyo ukuba isikhwama se-foil sonakalisiwe.Musa ukuphinda usebenzise iimvavanyo.
3. Isisombululo se-reagent ye-extraction iqulethe isisombululo setyuwa ukuba isisombululo sidibanisa ulusu okanye iliso, sihlambulule ngamanzi amaninzi.
4. Kuthintele ukungcoliseka ngokunqamlezayo kwesampulu ngokusebenzisa isikhongozeli esitsha sokuqokelelwa kwesampulu kumzekelo ngamnye ofunyenweyo.
5. Funda yonke inkqubo ngononophelo phambi kovavanyo.
6. Ungatyi, ungaseli okanye utshaye kwindawo apho imizekelo neekhithi ziphathwa khona.Phatha yonke imizekelo ngokungathi inee-arhente ezosulelayo.Qwalasela izilumkiso ezimiselweyo ngokuchasene nobungozi be-microbiological kuyo yonke inkqubo kwaye ulandele iinkqubo ezisemgangathweni zokulahlwa ngokufanelekileyo kwesampulu.Nxiba iimpahla ezikhuselayo ezifana needyasi zaselabhoratri, iiglavu ezilahlwayo kunye nokhuseleko lwamehlo xa imizekelo ivavanywa.
7. Ukuba usulelo nge-coronavirus yenoveli kurhanelwa ngokusekwe kwiikhrayitheriya zangoku zeklinikhi kunye ne-epidemiological screening ecetyiswa ngabasemagunyeni bezempilo yoluntu, iisampuli kufuneka ziqokelelwe kunye nezilumkiso ezifanelekileyo zokuthintela usulelo kwiinoveli coronavirus kwaye zithunyelwe kwisebe lezempilo likarhulumente okanye lengingqi ukuze kuhlolwe.Inkcubeko yentsholongwane akufuneki izame kwezi meko ngaphandle kokuba i-BSL 3+ iyafumaneka ukufumana kunye neesampulu zenkcubeko.
8. Musa ukutshintshiselana okanye udibanise ii-reagents ezivela kwiindawo ezahlukeneyo.
9. Ukufuma kunye nobushushu kunokuchaphazela kakubi iziphumo.
10. Izinto zokuvavanya ezisetyenzisiweyo kufuneka zilahlwe ngokuhambelana nemimiselo yendawo.
1. Ikhithi kufuneka igcinwe kwi-2-30 ° C de kube ngumhla wokuphelelwa kwexesha uprintwe kwisingxobo esivaliweyo.
2. Uvavanyo maluhlale kwisingxobo esitywiniweyo de lusetyenziswe.
3. Musa ukuba ngumkhenkce.
4. Kufuneka kuthathelwe ingqalelo ukukhusela amacandelo ekhithi ekungcoleni.Musa ukusebenzisa ukuba kukho ubungqina bokungcoliseka kwe-microbial okanye imvula.Ungcoliseko lwebhayoloji lwezixhobo zokukhupha, izikhongozeli okanye iirejenti kunokukhokelela kwiziphumo ezingezizo.
Qwalasela naziphi na izixhobo zemvelaphi yomntu njengezosulelo kwaye uziphathe usebenzisa iinkqubo eziqhelekileyo zokhuseleko lwendalo.
Igazi leCapillary lonke
Hlamba isandla sesigulana uze usivumele some.Hlaba isandla ngaphandle kokuchukumisa i-puncture.Gcoba ulusu nge-lancet enyumba.Sula uphawu lokuqala lwegazi.Hlikihla ngobunono isandla ukusuka esihlahleni ukuya entendeni ukuya emnweni ukwenza ithontsi legazi elingqukuva kwindawo yokuhlatywa.Yongeza iSampuli yeFingerstick Whole Blood kwisixhobo sovavanyo ngokusebenzisa ityhubhu yecapillary okanye amathontsi ajingayo.
i-venous Whole Blood:
Qokelela isampula yegazi kwi-lavender, eluhlaza okwesibhakabhaka okanye eluhlaza ityhubhu yokuqokelela phezulu (equlethe i-EDTA, i-citrate okanye i-heparin, ngokulandelanayo kwi-Vacutainer®) nge-veinpuncture.
I-Plasma
Qokelela isampula yegazi kwi-lavender, eluhlaza okwesibhakabhaka okanye eluhlaza ityhubhu yokuqokelela phezulu (equlethe i-EDTA, i-citrate okanye i-heparin, ngokulandelanayo kwi-Vacutainer®) nge-veinpuncture.Yahlula iplasma nge-centrifugation.Ukurhoxisa ngononophelo iplasma kwityhubhu ebhalwe kwangaphambili.
ISerum
Qokelela isampuli yegazi kwityhubhu ebomvu yokuqokelela phezulu (engenazo ii-anticoagulants kwi-Vacutainer®) nge-veinpuncture.Vumela igazi ukuba lihlule.Yahlula i-serum nge-centrifugation.Ukurhoxisa ngononophelo i-serum kwityhubhu entsha ebhalwe kwangaphambili.
Vavanya iisampuli ngokukhawuleza emva kokuqokelela.Gcina imizekelo kwi-2°C-8°C ukuba ayivavanywanga ngoko nangoko.
Gcina iisampuli kwi-2 ° C-8 ° C ukuya kwiintsuku ezi-5.Imizekelo kufuneka ifakwe emkhenkceni kwi -20°C ukuze igcinwe ixesha elide.
Kuphephe imijikelo emininzi yokunyibilikisa.Ngaphambi kovavanyo, zisa iisampulu ezikhenkcezisiweyo kwiqondo lobushushu begumbi ngokucothayo kwaye udibanise ngobunono.Iisampulu eziqulethe i-particle ebonakalayo kufuneka icaciswe nge-centrifugation ngaphambi kokuvavanya.Musa ukusebenzisa iisampulu ezibonisa i-gross lipemia, hemolysis enkulu okanye i-turbidity ukuze uthintele ukuphazamiseka ekutolikweni kweziphumo.
Zisa isampuli kunye nezixhobo zovavanyo kwiqondo lobushushu begumbi Xuba umboniso kakuhle phambi kokuba uvavanye xa unyibilikisiwe.Beka isixhobo sovavanyo kwindawo ecocekileyo nesicaba.
Kwisampulu yegazi ye-capillary:
Ukusebenzisa ityhubhu ye-capillary: Gcwalisa ityhubhu ye-capillary kunyeukudlulisela malunga ne-50µL (okanye amathontsi ama-2) wegazi elipheleleyo leminweumboniso kumfanekiso kakuhle (S) wesixhobo sovavanyo, uze wongezeUkuwisa oku-1 (malunga nama-30 µL)yeI-Diluent yesampulingoko nangoko kwisampulu kakuhle.
Kwisampulu yegazi elipheleleyo:
Gcwalisa idropper kunye nomzekelo ngokotshintshela amathontsi ama-2 (malunga nama-50 µL)yomzekelo kwiqula lesampulu.Ukuqinisekisa ukuba akukho amaqamza omoya.Emva kokogqithisela ithontsi enye (malunga nama-30 µL)yeSampuli Diluent ngoko nangoko kwisampulu equleni.
Kwisampulu yePlasma/Serum:
Gcwalisa idropper kunye nomzekelo ngokoUkutshintshela ithontsi enye (malunga nama-25 µL)yomzekelo kwiqula lesampulu.Ukuqinisekisa ukuba akukho amaqamza omoya.Emva kokogqithisela ithontsi enye (malunga nama-30 µL) yeSampuli Diluent ngoko nangoko kwisampulu equleni.
Seta isibali-xesha.Funda isiphumo ngemizuzu eyi-15.Ungafundi iziphumo emva20 imizuzu.Ukuze ugweme ukudideka, lahla isixhobo sokuvavanya emva kokutolika umphumo
| IZIPHUMO EZINYO:
| Ibhanti enye kuphela enemibala ibonakala kwindawo yokulawula (C).Akukho bhanti yemibala ebonakalayo ibonakala kwindawo yovavanyo (T). |
| IZIPHUMO EZINGAMBI:
| Iibhanti ezimbini ezinemibala zivela kwi-membrane.Elinye iqela livela kummandla wolawulo (C) kwaye elinye ibhendi libonakala kwindawo yovavanyo (T). |
| *QAPHELA: Ubunzulu bombala kumgca wovavanyo buya kwahluka ngokuxhomekeke kugxininiso lwezilwa-buhlungu ezibulalayo kwi-COVID-19 kumzekelo.Ngoko ke, nawuphi na umthunzi wombala kummandla wovavanyo kufuneka uthathwe njengento engalunganga. | |
|
IZIPHUMO EZINGAVUMI:
| Ibhendi yolawulo ayibonakali.Iziphumo zalo naluphi na uvavanyo olungakhange luvelise ibhendi yolawulo ngexesha lokufunda elichaziweyo mazityeshelwe.Nceda ujonge inkqubo kwaye uphinde ngovavanyo olutsha.Ukuba ingxaki iyaqhubeka, yeka ukusebenzisa ikhithi ngoko nangoko kwaye uqhagamshelane nomsasazi wakho wendawo. |
1. Ulawulo lwangaphakathi:Olu vavanyo luqulethe uphawu lolawulo olwakhelwe ngaphakathi, i-C band.Umgca we-C uphuhlisa emva kokongeza isampuli kunye nesampulu yokuxutywa.Ngaphandle koko, jonga yonke inkqubo kwaye uphinde uvavanyo ngesixhobo esitsha.
2. Ulawulo lwangaphandle:I-Good Laboratory Practice icebisa ukuba kusetyenziswe ulawulo lwangaphandle, olulungileyo kunye nolubi (olunikezelwe ngesicelo), ukuqinisekisa ukusebenza ngokufanelekileyo kovavanyo.
